Handling Database logins

I am writing an internet app and can access the DB in designmode just fine.

But in run-time, I get the error:
Login failed for user 'MYCOMPUTER\IUSR_MYCOMPUTER'
Where MYCOMPUTER is my username.

Now, I have a logon for this user for the DB with a blank password.

What am I doing worng here as fas as  giving my web app DB access?